This page will show two hypotethical cases: the first one will estimate the expected efficiency of a previously defined primer set; the second one will call to primer3 to design proper primers, which will be assessed for estimated efficiency.
This example will check for a known primer set. Don't mind about the primer design parameters above the 'sequence' text area: they won't be taken into account. Please press the 'send' button to execute this example.
In this case, we are interested in designing primers with 300 bp of amplicon length in which primers should have a melting temperature around 62 ªC. Moreover, they should have a 'C' or 'G' in their 3' terminus. Please press the 'send' button to execute it.