Tool description

pcrEfficiency is an open source tool which allows PCR efficiency prediction. Documentation and examples are available. Please cite pcrEfficiency: a Web tool for PCR amplification efficiency prediction BMC Bioinformatics 2011, 12:404 with doi:10.1186/1471-2105-12-404.

Efficiency calculator

Minimal information needed is your amplicon sequence; if you don't provide your primer sequences, several primers sorted by theoretical efficiency will be retrieved.

If forward and reverse primer sequences are included, expected efficiency will be calculated.

'Start' and 'end' refers to the subsequence which is liked to be amplified; if they are left blank, all the sequence will be taken into account.

Please fill out this form; 'Amplicon sequence' field is mandatory:
Sequence name:
Forward sequence:
Reverse sequence:
Amplicon sequence:

Optimum product size: Max Tm difference between primers:
Optimum primer Tm: Number of GCs flanking primer sequence:
Optimum primer length: Optimum primers GC content:
Salt concentration: DNA concentration:
Number of primer pairs to return: